如何通过数据挖掘，发现癌症治疗的靶标？

今天要介绍的这篇文章是我们中国人发表在ONCOLOGY REPORTS上的文章，ONCOLOGY REPORTS 2017年的影响因子为2.976，算是准三分的文章。

这篇文章的题目是：

Bioinformatic analysis suggests that UGT2B15 activates the Hippo‑YAP signaling pathway leading to the pathogenesis of gastric cancer

首先，第一步，作者在GEO数据库上下载了三个研究的数据：GSE54129，GSE79973，GSE56807，然后通过GEO数据库自带的工具GEO2R做差异表达分析，然后取交集的差异基因。

第二步，做GO富集分析

第三步，KEGG富集分析

第四步，PPI分析以及筛选hub基因

第五步，查看hub基因在各种条件的表达水平

第六步，进行生存分析

第七步，进行免疫组化检验

最后，作者得住结论：UGT2B15能作为癌症治疗的靶标。

附录原文的摘要：

Gastric cancer (GC) is one of the most common

malignancies that threatens human health. As the molecular

mechanisms unerlying GC are not completely understood,

identification of genes related to GC could provide new insights

into gene function as well as potential treatment targets. We

discovered that UGT2B15 may contribute to the pathogenesis

and progression of GC using GEO data and bioinformatic

analysis. Using TCGA data, UGT2B15 mRNA was found to be

significantly overexpressed in GC tissues; patients with higher

UGT2B15 had a poorer prognosis. It was further discovered that

UGT2B15 and FOXA1 were both upregulated, and UGT2B15

and Foxa1 were positively correlated in GC. It is known that

Foxa1 is a vital threshold to activate the Hippo‑YAP signaling

pathway. In addition, we suggest that a potential molecular

mechanisms includes UGT2B15 which may upregulate Foxa1,

activate the Hippo‑YAP signaling pathway and contribute to the

development of GC. Taken together, our findings demonstrate

that UGT2B15 may be an oncogene in GC and is a promising

therapeutic target for cancer treatment.