FCY1

Protein

Cytosine deaminase

Gene

FCY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of cytosine to uracil or 5-methylcytosine to thymine. Is involved in the pyrimidine salvage pathway, which allows the cell to utilize cytosine for pyrimidine nucleotide synthesis.2 Publications

Miscellaneous

Present with 5180 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

  • cytosine + H+ + H2O = NH4+ + uracil1 Publication

    EC:3.5.4.11 Publication

    Source: Rhea. « Hide
    cytosine

    ">zoom

    +
    H+
    +
    H2O
    =
    NH4+

    ">zoom

    +
    uracil

    ">zoom

Cofactori

Zn2+3 Publications

Kineticsi

kcat is 91 sec(-1) with cytosine as substrate and 17 sec(-1) with 5-fluorocytosine as substrate.1 Publication

  1. KM=1.1 mM for cytosine1 Publication
  2. KM=0.16 mM for 5-fluorocytosine1 Publication

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes uracil from cytosine.1 Publication1 Publication
Proteins known to be involved in this subpathway in this organism are:

  1. Cytosine deaminase (FCY1)

This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes uracil from cytosine, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature key Position(s) DescriptionActions Graphical view Length
Binding sitei 51 SubstrateCombined sources2 Publications 1
Metal bindingi 62 Zinc; catalyticCombined sources3 Publications 1
Active sitei 64 Proton donorCombined sources1 Publication 1
Metal bindingi 91 Zinc; catalyticCombined sources3 Publications 1
Metal bindingi 94 Zinc; catalyticCombined sources3 Publications 1
Binding sitei 155 SubstrateCombined sources2 Publications 1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular function Hydrolase
Ligand Metal-binding, Zinc

Enzyme and pathway databases

BioCyci MetaCyc:YPR062W-MONOMER
BRENDAi 3.5.4.1, 984
UniPathwayi UPA00574;UER00635

Names & Taxonomyi

Protein namesi
Recommended name:

Cytosine deaminase1 Publication (EC:3.5.4.11 Publication)

Short name:
yCD
Alternative name(s):
Cytosine aminohydrolase
Fluorocytosine resistance protein 11 Publication
Gene namesi
Name:FCY11 Publication
Ordered Locus Names:YPR062W
ORF Names:YP9499.17
Organismi Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri 559292 [NCBI]
Taxonomic lineagei Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
Proteomesi

Organism-specific databases

SGDi S000006266, FCY1
VEuPathDBi FungiDB:YPR062W

Subcellular locationi

  • UniProt annotation
  • GO - Cellular component

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Biotechnological usei

Also catalyzes the conversion of 5-fluorocytosine (5FC) to 5-fluorouracil (5FU); this activity allows the formation of a cytotoxic chemotherapeutic agent from a non-cytotoxic precursor. yCD/5FC is one of the most widely used enzyme/prodrug combinations for gene-directed enzyme prodrug therapy (GDEPT) for the treatment of cancers. 5FU is an anticancer drug used to treat breast, colon, rectal, stomach, and pancreatic cancers, and is the drug of choice for treating colorectal carcinoma. However, the drug has high gastrointestinal and hematological toxicities. In contrast, the prodrug 5FC is fairly non-toxic to human, because of the lack of CD activity in human cells. By producing 5FU in the tumor, the CD/5FC system minimizes the undesired toxic effects of 5FU.1 Publication

PTM / Processingi

Molecule processing

Feature key Position(s) DescriptionActions Graphical view Length
ChainiPRO_0000171702 1 – 158 Cytosine deaminaseAdd BLAST 158

Proteomic databases

MaxQBi Q12178
PaxDbi Q12178
PRIDEi Q12178

PTM databases

iPTMneti Q12178

Interactioni

Subunit structurei

Homodimer.

1 Publication

Protein-protein interaction databases

BioGRIDi 36235, 61 interactors
DIPi DIP-1662N
IntActi Q12178, 2 interactors
MINTi Q12178
STRINGi 4932.YPR062W

Miscellaneous databases

RNActi Q12178, protein

Structurei

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

3D structure databases

SMRi Q12178
ModBasei Search...
PDBe-KBi Search...

Miscellaneous databases

EvolutionaryTracei Q12178

Family & Domainsi

Domains and Repeats

Feature key Position(s) DescriptionActions Graphical view Length
Domaini 9 – 129 CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST 121

Compositional bias

Feature key Position(s) DescriptionActions Graphical view Length
Compositional biasi 129 – 132 Poly-Val 4

Sequence similaritiesi

Phylogenomic databases

HOGENOMi CLU_025810_7_2_1
InParanoidi Q12178
OMAi AILHAEM

Family and domain databases

InterProi View protein in InterPro
IPR016192, APOBEC/CMP_deaminase_Zn-bd
IPR002125, CMP_dCMP_dom
IPR016193, Cytidine_deaminase-like
Pfami View protein in Pfam
PF00383, dCMP_cyt_deam_1, 1 hit
SUPFAMi SSF53927, SSF53927, 1 hit
PROSITEi View protein in PROSITE
PS00903, CYT_DCMP_DEAMINASES_1, 1 hit
PS51747, CYT_DCMP_DEAMINASES_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q12178-1 [UniParc]FASTAAdd to basket

« Hide

10         20         30         40         50MVTGGMASKW DQKGMDIAYE EAALGYKEGG VPIGGCLINN KDGSVLGRGH         60         70         80         90        100NMRFQKGSAT LHGEISTLEN CGRLEGKVYK DTTLYTTLSP CDMCTGAIIM        110        120        130        140        150YGIPRCVVGE NVNFKSKGEK YLQTRGHEVV VVDDERCKKI MKQFIDERPQ 

DWFEDIGE
Length:158
Mass (Da):17,507
Last modified:November 1, 1996 - v1
Checksum:i19DB41E9AF26ADDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi		 U55193 Genomic DNA Translation: AAC13409.1
AF005261 Genomic DNA Translation: AAB67713.1
Z71255 Genomic DNA Translation: CAA95006.1
Z49219 Genomic DNA Translation: CAA89179.1
BK006949 Genomic DNA Translation: DAA11483.1
PIRi S54083
RefSeqi NP_015387.1, NM_001184159.1

Genome annotation databases

EnsemblFungii YPR062W_mRNA; YPR062W; YPR062W
GeneIDi 856175
KEGGi sce:YPR062W

Similar proteinsi

  • 100% Identity
  • 90% Identity
  • 50% Identity

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi		 U55193 Genomic DNA Translation: AAC13409.1
AF005261 Genomic DNA Translation: AAB67713.1
Z71255 Genomic DNA Translation: CAA95006.1
Z49219 Genomic DNA Translation: CAA89179.1
BK006949 Genomic DNA Translation: DAA11483.1
PIRi S54083
RefSeqi NP_015387.1, NM_001184159.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji		 PDB entry Method Resolution (Å) Chain Positions PDBsum
1OX7 X-ray 1.43 A/B 1-158 [»]
1P6O X-ray 1.14 A/B 1-158 [»]
1RB7 X-ray 2.10 A/B 1-158 [»]
1UAQ X-ray 1.60 A/B 1-158 [»]
1YSB X-ray 1.70 A/B 1-158 [»]
1YSD X-ray 1.90 A/B 1-158 [»]
2O3K X-ray 2.30 A/B 1-158 [»]
SMRi Q12178
ModBasei Search...
PDBe-KBi Search...

Protein-protein interaction databases

BioGRIDi 36235, 61 interactors
DIPi DIP-1662N
IntActi Q12178, 2 interactors
MINTi Q12178
STRINGi 4932.YPR062W

PTM databases

iPTMneti Q12178

Proteomic databases

MaxQBi Q12178
PaxDbi Q12178
PRIDEi Q12178

Genome annotation databases

EnsemblFungii YPR062W_mRNA; YPR062W; YPR062W
GeneIDi 856175
KEGGi sce:YPR062W

Organism-specific databases

SGDi S000006266, FCY1
VEuPathDBi FungiDB:YPR062W

Phylogenomic databases

HOGENOMi CLU_025810_7_2_1
InParanoidi Q12178
OMAi AILHAEM

Enzyme and pathway databases

UniPathwayi UPA00574;UER00635
BioCyci MetaCyc:YPR062W-MONOMER
BRENDAi 3.5.4.1, 984

Miscellaneous databases

EvolutionaryTracei Q12178
PROi PR:Q12178
RNActi Q12178, protein

Family and domain databases

InterProi View protein in InterPro
IPR016192, APOBEC/CMP_deaminase_Zn-bd
IPR002125, CMP_dCMP_dom
IPR016193, Cytidine_deaminase-like
Pfami View protein in Pfam
PF00383, dCMP_cyt_deam_1, 1 hit
SUPFAMi SSF53927, SSF53927, 1 hit
PROSITEi View protein in PROSITE
PS00903, CYT_DCMP_DEAMINASES_1, 1 hit
PS51747, CYT_DCMP_DEAMINASES_2, 1 hit
ProtoNeti Search...
MobiDBi Search...

Entry informationi

Entry namei FCY1_YEAST
Accessioni Primary (citable) accession number: Q12178
Secondary accession number(s): D6W467
Entry historyi Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 10, 2021
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusi Reviewed (UniProtKB/Swiss-Prot)
Annotation program Fungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families
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