修饰引物选择指南

表1 荧光基团的激发、发射波长及推荐的淬灭基团

发射光颜色	Dye	Abs (nm)	Em (nm)	Taqman 对应淬灭基团	MGB探针 淬灭基因	分子信标 淬灭基因
	AMCA	350	440
	Atto425	436	484	BHQ-1		Dabcyl
	Alexa Fluor 488	490	525	BHQ-1	MGB	Dabcyl
	6-FAM	494	518	BHQ-1	MGB	Dabcyl
	TET	521	536	BHQ-1	MGB	Dabcyl
	JOE	520	548	BHQ-1	MGB	Dabcyl
	VIC	538	554	BHQ-1	MGB	Dabcyl
	HEX	535	556	BHQ-1		Dabcyl
	NED	546	575	BHQ-2
	Cy3	552	570	BHQ-2
	TAMRA	565	580	BHQ-2
	ROX	585	605	BHQ-2
	Texas red	595	615	BHQ-2
	Cy5	643	667	BHQ-3
	Quasar 670	647	667	BHQ-3
	Cy5.5	684	710	BHQ-3
	Cy7	749	767

备注：上方波长数据为理论值，在不同的Oligo中，波长可能发生不同程度的偏移

表2 常用荧光定量PCR仪做多重qPCR检测时支持的荧光基团

仪器型号	染料1	染料2	染料3	染料4	染料5
Agilent MX3000P/3005P/4000	FAM	HEX/VIC/JOE/TET	Cy3	Texas red/TEX-615	Cy5
ABI 7300	FAM	HEX/VIC/JOE
ABI 7500 FAST	FAM	HEX/VIC/JOE	Cy3	Texas red/TEX-615	Cy5
ABI 7900 HT	FAM	HEX/VIC/JOE/TET
ABI QuantStudio?系列	FAM	HEX/VIC/JOE/TET	Cy3	Texas red/TEX-615	Cy5
ABI StepOne	FAM	HEX/VIC/JOE/TET
ABI StepOnePlus	FAM	HEX/VIC/JOE	TAMRA
ABI ViiA?7	FAM	HEX/VIC/JOE/TET		Texas red/TEX-615	Cy5
Bio-Rad CFX 96/384	FAM	HEX/VIC/JOE/TET		Texas red/TEX-615	Cy5
Bio-Rad iCycler	FAM	HEX/VIC/JOE/TET	Cy3	Texas red/TEX-615	Cy5
Bio-Rad QX200	FAM	HEX/VIC/JOE
Roche LightCycler 96	FAM	HEX/VIC/JOE		Texas red/TEX-615	Cy5
Roche LightCycler 480	FAM	HEX/VIC/JOE/TET		Texas red/TEX-615	Cy5